Journal: Journal of Pain Research

Article Title: Wrist-Ankle Acupuncture Alleviates Paclitaxel-Induced Neuropathic Pain in Mice by Neuronal α7 Nicotinic Acetylcholine Receptor–Dependent Modulation of Spinal Glutamatergic/NMDAR Signaling

doi: 10.2147/JPR.S604818

Figure Lengend Snippet: WAA alleviates paclitaxel-induced mechanical allodynia and thermal hyperalgesia in a neuronal α7nAChR–dependent manner. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. ( a ) Experimental design and timeline for the Chrna7 flox/flox mouse cohort; detailed procedures are provided in the Methods. ( b ) Representative Western blot bands of α7nAChR in the spinal dorsal horn. ( c ) Quantification of α7nAChR protein levels 21 days after lumbar dorsal horn microinjection of control virus or rAAV-hSyn-SV40-NLS-Cre (N=3, t = 3.2, P = 0.033). ( d ) Representative immunofluorescence images showing colocalization of α7nAChR (red) and NeuN (green); scale bar: 100 μm. ( e ) Quantification of neuronal association/targeting using Manders’ overlap coefficient (fraction of α7nAChR overlapping NeuN, N=3, t = 41.833, P = 0.001). ( f ) Behavioral assessments of mechanical (paw withdrawal threshold, PWT) and thermal sensitivity (paw withdrawal latency, PWL) at days 0, 7, and 14; WAA-induced analgesia was attenuated after dorsal horn neuronal α7nAChR knockdown (N=9,day 14: F PWT =342.009, P PWT < 0.001; F PWL =333.245, P PWL < 0.001). ( g ) Representative Western blot bands of α7nAChR in the spinal dorsal horn across indicated groups following paclitaxel administration and WAA treatment. ( h ) Densitometric quantification of α7nAChR protein levels corresponding to panel ( g ) (N=3, F =46.729, P < 0.001). ( i ) Representative immunofluorescence images of α7nAChR (red) and NeuN (green) in the spinal cord dorsal horn on day 14 after model induction; scale bar: 100 μm. ( j ) Quantification of α7nAChR immunofluorescence intensity (mean intensity), corresponding to panel ( i ) (N=3, F =81.123, P < 0.001).

Article Snippet: Except for the saline group, paclitaxel (PTX)-induced CIPN was established as previously described by Toma et al Briefly, a 5 mg/mL PTX (MedChemExpress) stock solution was prepared in 10% DMSO and 90% (20% sulfobutylether-β-cyclodextrin [SBE-β-CD] in saline; MedChemExpress).

Techniques: Western Blot, Microinjection, Control, Virus, Immunofluorescence, Knockdown

Journal: Journal of Pain Research

Article Title: Wrist-Ankle Acupuncture Alleviates Paclitaxel-Induced Neuropathic Pain in Mice by Neuronal α7 Nicotinic Acetylcholine Receptor–Dependent Modulation of Spinal Glutamatergic/NMDAR Signaling

doi: 10.2147/JPR.S604818

Figure Lengend Snippet: Intrathecal NMDAR activation attenuates the analgesic and spinal molecular effects of WAA in paclitaxel-induced CIPN. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. ( a ) Experimental design and timeline for the C57BL/6 cohort; detailed procedures are provided in the Methods. ( b ) Behavioral assessment of PWT and PWL at days 0, 7, and 14. Intrathecal administration of an NMDAR agonist prior to WAA reduced the behavioral analgesic benefit of WAA. (N=9; two-way repeated-measures ANOVA; day 14: F PWT =281.188, P PWT < 0.001; F PWL =461.233, P PWL < 0.001). ( c ) Glutamate (glutamic acid) content in the spinal dorsal horn. (N=3, F =54.286, P < 0.001). ( d ) Representative Western blot bands of proteins associated with glutamatergic/NMDAR pathway activity in the spinal dorsal horn. ( e – i ) Densitometric quantification of NR2B ( e ) VGluT2 ( f ) p-JNK ( g ) p-CREB ( h ) and CGRP ( i ) in the spinal cord dorsal horn.(N=3, NR2B: F =11.877, P < 0.01; VGluT2: F =36.553, P < 0.001; p-JNK: F =8.216, P < 0.01; p-CREB: F =16.911, P < 0.01; CGRP: F =25.487, P < 0.001).

Article Snippet: Except for the saline group, paclitaxel (PTX)-induced CIPN was established as previously described by Toma et al Briefly, a 5 mg/mL PTX (MedChemExpress) stock solution was prepared in 10% DMSO and 90% (20% sulfobutylether-β-cyclodextrin [SBE-β-CD] in saline; MedChemExpress).

Techniques: Activation Assay, Western Blot, Activity Assay

Journal: Journal of Pain Research

Article Title: Wrist-Ankle Acupuncture Alleviates Paclitaxel-Induced Neuropathic Pain in Mice by Neuronal α7 Nicotinic Acetylcholine Receptor–Dependent Modulation of Spinal Glutamatergic/NMDAR Signaling

doi: 10.2147/JPR.S604818

Figure Lengend Snippet: α7nAChR in VGluT2-expressing glutamatergic neurons is required for the analgesic and spinal glutamatergic/NMDAR-modulatory effects of WAA in paclitaxel-induced CIPN. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. ( a ) Experimental design and timeline for VGluT2 neuron–specific Chrna7 conditional knockout mice ( Chrna7 flox/flox ;Slc17a6 -Cre + , α7-cKO(VGluT2)) and littermate Cre-negative controls ( Chrna7 flox/flox ;Slc17a6 -Cre − ); detailed procedures are provided in the Methods. ( b ) Representative Western blot bands of α7nAChR in the spinal dorsal horn. ( c ) Densitometric quantification of α7nAChR protein levels in α7-cKO(VGluT2) mice and controls (N=3, t =9.954, P =0.001). ( d ) Representative immunofluorescence images of the spinal dorsal horn showing α7nAChR (red) and VGluT2 (green); scale bar, 100 μm. ( e ) Quantification of α7nAChR immunofluorescence intensity (mean intensity) corresponding to panel ( d ). (N=3, t =33.319, P < 0.001). ( f ) Behavioral assessment of PWT and PWL at days 0, 7, and 14. VGluT2 neuron–specific α7nAChR deletion attenuated the analgesic effects of WAA (N=9; two-way repeated-measures ANOVA; day 14: F PWT =308.427, P PWT < 0.001; F PWL =301.306, P PWL < 0.001). ( g ) Glutamate (glutamic acid) content in the spinal dorsal horn. (N=3, F =37.871, P <0.001). ( h )Representative Western blot bands of proteins associated with glutamatergic/NMDAR pathway activity in the spinal dorsal horn. ( i – m ) Densitometric quantification of NR2B ( i ) VGluT2 ( j ) p-JNK ( k ) p-CREB ( l ) and CGRP ( m ) in the spinal dorsal horn (N=3, NR2B: F =36.601, P < 0.001; VGluT2: F =11.265, P < 0.001; p-JNK: F =21.336, P < 0.001; p-CREB: F =13.144, P < 0.01; CGRP: F =15.896, P < 0.001).

Article Snippet: Except for the saline group, paclitaxel (PTX)-induced CIPN was established as previously described by Toma et al Briefly, a 5 mg/mL PTX (MedChemExpress) stock solution was prepared in 10% DMSO and 90% (20% sulfobutylether-β-cyclodextrin [SBE-β-CD] in saline; MedChemExpress).

Techniques: Expressing, Knock-Out, Western Blot, Immunofluorescence, Activity Assay

Journal: Discover Oncology

Article Title: FABP5 confers resistance to drug-induced ROS toxicity in cervical cancer cell lines by suppressing the PPARγ/CPT1A signaling pathway

doi: 10.1007/s12672-026-05268-7

Figure Lengend Snippet: FABP5 in cervical cancer cells can promote resistance to CDDP and PTX to reduce apoptosis. A , B IC 50 of cervical cancer stable cells at different CDDP and PTX concentrations, ( n = 4). C , D Apoptosis assay in stable cells treated with CDDP at different concentrations. E , F Analysis of early apoptosis in stable cells after exposure to varying concentrations of CDDP, ( n = 3). G , H Apoptosis assay in stable cells treated with PTX at different concentrations. I , J Analysis of early apoptosis in stable cells after exposure to varying concentrations of PTX, ( n = 3). ns: no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001

Article Snippet: CDDP (HY−17394) and PTX (HY-B0015) were purchased from MCE ( https://www.medchemexpress.cn/ ).

Techniques: Apoptosis Assay

Journal: Discover Oncology

Article Title: FABP5 confers resistance to drug-induced ROS toxicity in cervical cancer cell lines by suppressing the PPARγ/CPT1A signaling pathway

doi: 10.1007/s12672-026-05268-7

Figure Lengend Snippet: FABP5 Confers Resistance to CDDP and PTX in Cervical Cancer Cells by modulating ROS. A , B Fluorescence images of ROS generated by cells in different groups under varying concentrations of CDDP. C , D Flow cytometry results showing the proportion of ROS-producing cells in different groups under varying CDDP concentrations. E , F Fluorescence images showing ROS production in cells from different groups treated with PTX at various concentrations. G , H Flow cytometry results showing the proportion of ROS-producing cells in different groups treated with PTX at various concentrations

Article Snippet: CDDP (HY−17394) and PTX (HY-B0015) were purchased from MCE ( https://www.medchemexpress.cn/ ).

Techniques: Fluorescence, Generated, Flow Cytometry

Journal: Discover Oncology

Article Title: FABP5 confers resistance to drug-induced ROS toxicity in cervical cancer cell lines by suppressing the PPARγ/CPT1A signaling pathway

doi: 10.1007/s12672-026-05268-7

Figure Lengend Snippet: ROS induced by CDDP and PTX were independent of the type of drug. A , B Cell cycle assay of stable cells without drug interference, ( n = 3). C , D Cell cycle assay after CDDP treatment at different concentrations, ( n = 3). E , F Cell cycle assay after PTX treatment at different concentrations, ( n = 3). ns: no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001

Article Snippet: CDDP (HY−17394) and PTX (HY-B0015) were purchased from MCE ( https://www.medchemexpress.cn/ ).

Techniques: Cell Cycle Assay

Journal: Discover Oncology

Article Title: FABP5 confers resistance to drug-induced ROS toxicity in cervical cancer cell lines by suppressing the PPARγ/CPT1A signaling pathway

doi: 10.1007/s12672-026-05268-7

Figure Lengend Snippet: Drug-induced ROS can activate the PPARγ/CPT1A signaling pathway. A ORO staining of the lipid in OEFABP5 cells afrer CDDP treatment. B ORO staining of the lipid in shNC cells afrer CDDP or PTX treatment. C Western blot of PPARγ, CPT1A, ATP5A1 protein expression in cells in shNC and OEFABP5 cells afrer CDDP or PTX treatment ( n = 3)

Article Snippet: CDDP (HY−17394) and PTX (HY-B0015) were purchased from MCE ( https://www.medchemexpress.cn/ ).

Techniques: Staining, Western Blot, Expressing